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Objective To explore the feasibility and clinical efficacy of entirely mastoscopic axillary lymph node dissection for patients with breast cancer. Methods To analyze the clinical data and the procedure of the Breast cancer cases with entirely mastescopic axillary lymph node dissection. Results All the operations of those cases re-ceived entirely mastoseopic axillary lymph nodes dissection were successful, the average operating duration was 97min,and average of 5 ～ 14 lymph nodes were removed;2 cases had the positive lymph node. The procedure of those operations went well ,and there was no operation complication. After the operation the shoulder mobilization had unlim-ited, and no cancer cell was transplanted in operations holes. Conclusion Entirely mastoscopic axillary lymph node dissection can be carried out in grass-root hospitals and can be taken as one of the usual operation methods.
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Objective To analyze the outcome in advanced age patients with obstruction of the left colonrectal cancer treated by intraoperative cavage method.Methods From 1998 and 2004,37 senile patients with acute obstruction for cancer underwent surgery.The patients were treated with intraoperative lavage,resection and primary anastomosis.Results All patients,no anastomotic leakage occurred.The patients got a well recovery post-operation.Conclusion One-stage emergency surgery for left obstructing colonic cancers is safe with the regard of intraoperative lavagation methods.
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Objective To observe the expression law of cytokine signaling suppressors (SOCSs) mRNA in burn rats with Staphylococcus aureus sepsis and investigate their potential role in the pathogenesis of postburn sepsis. Methods Wistar rats were inflicted with 20% TBSA Ⅲ? scald, followed by Staphylococcus aureus challenge. Then, the expressions of SOCS1, SOCS2 and SOCS3 mRNA and interferon-? (IFN-?) levels in the liver and lungs were determined. Results With Staphylococcus aureus challenge after burn, IFN-? levels in the liver and lungs were significantly elevated and reached peak at the 0.5th and 6th hours, respectively (P
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<p><b>OBJECTIVE</b>To investigate the protective effect and its underlying mechanism of 2,4-diamino-6-hydroxy-pyrimidine (DAHP), an inhibitor of GTP-cyclohydrolase I (GTP-CHI), on postburn Staphylococcus aureus (S. aureus) sepsis in rats.</p><p><b>METHODS</b>Fifty-six Wistar rats were randomly divided into four groups, i.e. normal control, scalding control, postburn sepsis group and DAHP treatment group. Tissue samples from liver, kidneys, lungs and heart were aseptically taken, and in which the GTP-CHI and inducible nitric oxide synthase (iNOS) contents and the mRNA expression of tumor necrosis factor-alpha (TNFalpha) were determined. Furthermore, biopterin (BH(4)) and nitric oxide (NO) levels in these tissue were also measured.</p><p><b>RESULTS</b>After the scalding injury followed by bacterial challenge, the GTP-CHI gene expression and biopterin levels were significantly increased in all tissue sampled, and so were iNOS mRNA expression and NO (P < 0.01), especially in liver and lungs. The expressions of GTP-CHI mRNA and iNOS mRNA and the production of BH(4) and NO in all tissue were evidently inhibited by the pretreatment with DAHP (P < 0.05 approximately 0.01). At the same time, the TNFalpha expression was also obviously decreased. In addition, The mortality at 6 hr in rats of DAHP treatment group was decreased.</p><p><b>CONCLUSION</b>The prognosis of the scalding rats complicated by sepsis caused by G(+) bacteria could be improved by DAHP pretreatment, which might be related to the inhibition of the production of BH(4) and NO by DAHP.</p>
Subject(s)
Animals , Male , Rats , Biopterins , Metabolism , Burns , Genetics , Metabolism , Enzyme Inhibitors , Pharmacology , GTP Cyclohydrolase , Genetics , Gene Expression Regulation , Heart , Hypoxanthines , Pharmacology , Kidney , Metabolism , Liver , Metabolism , Lung , Metabolism , Myocardium , Metabolism , Nitric Oxide , Metabolism , Nitric Oxide Synthase , Genetics , RNA, Messenger , Genetics , Metabolism , Rats, Wistar , Sepsis , Staphylococcal Infections , Staphylococcus aureus , Sugar Acids , Time Factors , Tumor Necrosis Factor-alpha , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To investigate the potential role and changes in CD14 in postburn Staphylococcus aureus sepsis.</p><p><b>METHODS</b>Wistar rats were subjected to a 20% total body surface area (TBSA) full-thickness thermal injury and followed by Staphylococcus aureus challenge. Plasma and tissue samples from liver, kidneys, lungs and heart were collected to determine endotoxin (lipopolysaccharide, LPS) levels and CD14 mRNA expression.</p><p><b>RESULTS</b>It was found that after thermal injury combined with Staphylococcus aureus sepsis, LPS levels in various tissues were markedly increased, peaking at 2 approximately 6 h (P < 0.05), then returned to baseline at 24 h. Plasma LPS level was also increased significantly at 2hrs after Staphylococcus aureus challenge compared with that in normal control animals (0.3056 EU/ml vs. 0.1250 EU/ml, P < 0.05). In addition, the intestinal diamine oxidase (DAO) activity decreased (P < 0.05). This indicated that the integrity of intestinal mucosa was damaged. Meanwhile, tissue CD14 mRNA expressions were up-regulated in all tissues (P < 0.05), especially in lungs.</p><p><b>CONCLUSION</b>These data suggested that gut-originated LPS translocation and CD14 mRNA expression could be induced by thermal injury with Staphylococcus aureus sepsis. The up-regulation of CD14 gene expression might be associated with the local endotoxin activation secondary to postburn sepsis.</p>
Subject(s)
Animals , Male , Rats , Burns , Genetics , Gene Expression Regulation , Kidney , Metabolism , Lipopolysaccharide Receptors , Genetics , Lipopolysaccharides , Blood , Metabolism , Liver , Metabolism , Lung , Metabolism , Myocardium , Metabolism , RNA, Messenger , Genetics , Metabolism , Rats, Wistar , Sepsis , Genetics , Staphylococcal Infections , Genetics , Staphylococcus aureus , Time Factors , Up-RegulationABSTRACT
AIM: To investigate the role of staphylococcal enterotoxin B (SEB) in early intestinal injury in scald rats with Staphylococcus aureus sepsis. METHODS: 86 male Wistar rats were randomly divided into four groups as follows: normal controls ( n= 10), scald control group( n= 10), postburn sepsis group( n= 50) and SEB monoclonal antibody (MAb)treatment group ( n= 16). Plasma samples were collected to determine SEB, endotoxin, tumor necrosis factor-? (TNF-?) and interferon-? (IFN-?). RESULTS: After scald injury followed by Staphylococcus aureus challenge, the levels of SEB, TNF-? and IFN-? in plasma were significantly higher than those of normal controls, peaking at 2-6 h ( P
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A new animal model of multiple system organ failure (MSOF) in rabbits is used in this experiment. The purpose of the current study is to observe dynamically the changes in the intestinal flora, the relationship between the changes and bacterial translocation to various organs and endotoxemia. The results show that there is no obvious alteration in population of Bacteroides, but a significant increase in enteric bacilli population in the contents of the ileocecum is found. Changes in the intestinal micro-flora correlate to the positive bacterial culture of the blood, viscera, and the level of endotoxin in the plasma. The results of our work suggest that ecological imbalance in the intestinal flora may promote-the gut-derived septic process and the development of MSOF.
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To determine the role of biopterin in acute lung injury in scald rats with infection, and to observe the therapeutic effect of GTP-cyclohydrolase I(GTP-CHI) inhibitor on postburn sepsis, 56 male Wistar rats were randomly divided into four groups: normal controls (n=10), scald control group(n=10), postburn sepsis group(n=20) and 2,4-diamino-6-hydroxy-pyrimidine (DAHP) treatment group (n=16). Tissue samples from lungs were collected to determine GTP-CHI and inducible nitric oxide synthase (iNOS) mRNA expression, meanwhile biopterin, nitric oxide (NO), tumor necrosis factor-?(TNF-?) and interferon-? (IFN-?) levels in lungs were also measured. The results showed that after scald injury followed by Staphylococcus aureus challenge, GTP-CHI gene expression and biopterin levels significantly elevated in lungs, so did the values of iNOS mRNA expression and NO induction (P